SAGE | Serial Analysis of Gene Expression
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SAGE | Serial Analysis of Gene Expression
SAGE (Serial Analysis of Gene Expression) is a molecular technique used to get information about the transcription profile. The mRNA is converted into cDNA and short fragments of the cDNA are self ligated to form a long chain, which is finally ligated into a plasmid. The insert present in the plasmid is amplified by PCR and sent for DNA ...
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