8.10: Sanger Sequencing of DNA
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A researcher used the Sanger method to sequence the DNA fragment shown. The red asterisk indicates a fluorescent label.*5'3'3'-OHATTACGCAAGGACATTAGAC—5'She reacted a sample of the DNA with DNA polymerase and four different nucleotide mixtures (in an appropriate buffer). Someof the mixtures included dideoxynucleotides (ddNTPs) in relatively small amounts.The reseacher then separated the resulting DNA by electrophoresis on a polyacrylamide gel and located the fluorescent bands onthe gel. The image
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