For DFM, 20 µL of the supernatant were transferred into a new, clean slide and a cover slip was placed over it. The preparation was examined under a dark field microscope (Carl Zeiss, Germany). The ...
O trabalho tem como objetivo analisar as associações de variantes polimórficas dos genes PRL e BLG com resistência e suscetibilidade à mastite em vacas holandesas. O estudo experimental consistiu na ...
We detected the exon 18 mutation using standard PCR and RFLP methods (available on request). We used the Woolf method 3 to test for association in the combined data set and MAPMAKER/SIBS (ref. 4) to ...
Abstract: Polymerase chain reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) is an established molecular biology technique leveraging DNA sequence variability for organism identification, ...